Chlorine Equivalency Test

Test Objective:
To determine the equivalent concentration of SyDERMA® necessary to be as effective as available chlorine to kill bacteria.

Test Method:
The standard test procedure described in the Official Methods of Analysis of the Association of Official Analytical Chemistry International, 16th Ed., Vol. 1, Locator 6.3.01, AOAC Official Method 955.16 Chlorine (Available) in Equivalent Concentration was used to determine the chlorine equivalency of SyDERMA®. This test procedure test both chlorine (NaOCl) and SyDERMA® against Staphylococcus aureus (ATCC No. 6538).

Interpretation of Assay:
The results of the chlorine equivalency test are expressed as the equivalent parts per million (PPM) needed of the test product to prohibit bacterial growth compared to that of available chlorine in various concentrations.

Test Results:
SyDERMA®,when tested in accordance with standard testing procedures, was found to be equivalent to available Chlorine (NaOCl) at the following concentrations:

• 50 PPM concentration of SyDERMA is equivalent to 200 PPM concentration of Chlorine.
  
• 35 PPM concentration of SyDERMA is equivalent to 100 PPM concentration of Chlorine.
  
• 25 PPM concentration of SyDERMA is equivalent to 50 PPM concentration of Chlorine.
  

Observations:

Chlorine Equivalent Concentration against Staphylococcus aureus (ATCC No. 6538)

Subculture Series

Test Solution Concentration (Parts per Million of Available NaOCL) 1 2 3 4 5 6 7 8 9 10 NaOCL 200 - - - - - + + + + + SyDERMA® 50 - - - - - + + + + +                         NaOCL 100 - - - + + + + + + + SyDERMA® 35 - - - + + + + + + +                         NaOCL 50 - - + + + + + + + + SyDERMA® 25 - - + + + + + + + +

"-" indicates no bacterial growth "+" indicates no bacterial growth