Phenol Coefficient Test

Test Objective:
To determine the effectiveness of SyDERMA® relative to phenol to kill bacteria.

Test Method:
The standard test procedure described in the Official Methods of Analysis of the Association of Official Analytical Chemistry International, 16th Ed., Vol. 1, Locator 6.1.02, AOAC Official Method 955.12 Phenol Coefficient Methods Testing was used to determine the phenol coefficient of SyDERMA.. This test procedure test both phenol and SyDERMA. against Staphylococcus aureus (ATCC No. 6538).

Interpretation of Assay:
The phenol coefficient is the value obtained by dividing the highest dilution of the test solution by the highest dilution of phenol that sterilizes the given culture of bacteria under standard conditions of time and temperature.

Test Results:
SyDERMA®, when tested in accordance with standard testing procedures was found to have a phenol coefficient of 5.26.

Observations:

Phenol Coefficient against Staphylococcus aureus (ATCC No. 6538)

SyDERMA® Bacterial Activity

Dilution 5 minutes 10 minutes 15 minutes 1 to 10 No Growth No Growth No Growth 1 to 50 No Growth No Growth No Growth 1 to 100 No Growth No Growth No Growth 1 to 500 Growth No Growth No Growth 1 to 640 Growth Growth No Growth

Phenol Bacterial Activity

Dilution 5 minutes 10 minutes 15 minutes 1 to 90 No Growth No Growth No Growth 1 to 95 Growth No Growth No Growth 1 to 100 Growth Growth No Growth

At the 10-minute mark, SyDERMA® exhibited no bacterial growth at a dilution of 1 to 500. Phenol exhibited the same no bacterial growth characteristic at the 10-minute mark at a dilution of 1 to 95. A dilution of 1 part SyDERMA® in 500 parts of dilution has the same sterilizing characteristics as phenol at a dilution of 1 to 95. Therefore, the phenol coefficient of SyDERMA® is 500÷95=5.26.