Zone of Inhibition Test

Test Objective:
To determine the effectiveness of SYDERMA® to provide a zone of inhibition against certain bacteria.

Test Method:
Sample cultures of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Streptococcus pyogenes, Acinetobacter antritus, and Candida albicans bacteria were prepared in concentrations ranging from 9.3 x 109 to 11.2 x 109. The procedures used were in accordance with the Official Methods of Analysis of the Association of Official Analytical Chemistry, Chapter 6. The organisms tested are indicated in Federal Register, Vol. 55/No. 140, Monday, July 22, 1991. The zone of inhibition measurements were taken after twenty-four (24) hours.

Interpretation of Assay:
An area of no-growth greater than 6 mm in a bacteria's plate culture would indicate that the tested product is an effective growth inhibitor against that bacteria.

Test Results:
SyDERMA®, when tested in accordance with the Official Methods of Analysis of the Association of Official Analytical Chemistry, Chapter 6, was found to be effective against the organisms tested under the Zone of Inhibition Test.

Observations:

Bacteria	Culture Concentration	Zone of Inhibition
Acinetobacter antritus	9.3 x 10 9	9.9mm
Candida albicans	10.0 x 10 9	7.8mm
Escherichia coli	10.4 x 10 9	16.3mm
Pseudomonas aeruginosa	11.2 x 10 9	12.1mm
Staphylococcus aureus	10.7 x 10 9	12.7mm
Streptococcus pyogenes	9.5 x 10 9	11.9mm